{"id":2559,"date":"2020-11-15T12:28:16","date_gmt":"2020-11-15T12:28:16","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2559"},"modified":"2020-11-15T12:28:16","modified_gmt":"2020-11-15T12:28:16","slug":"anti-human-cd32-antibody-clone-iv-3-mouse-monoclonal-igg2b-antibody-against-human-cd32","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/anti-human-cd32-antibody-clone-iv-3-mouse-monoclonal-igg2b-antibody-against-human-cd32\/","title":{"rendered":"Anti-Human CD32 Antibody, Clone IV.3 Mouse monoclonal IgG2b antibody against human CD32"},"content":{"rendered":"

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Overview<\/h3>\n
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The IV.3 antibody reacts with human CD32 (Fc\u03b3RII), an ~40 kDa type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, immunomodulation and platelet aggregation. CD32 is encoded by three genes (A, B, C) and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. All isoforms are expressed by monocytes\/macrophages, placental trophoblasts and endothelial cells. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. Isoform IIc is expressed by NK cells and neutrophils. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes. These interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the IV.3 antibody may be employed as a blocking antibody to reduce non-specific binding. The IV.3 antibody binds most strongly to the IIa isoforms of CD32, with the epitope mapped to amino acids 132 – 137 [FSHLDP] in domain 2, within the ligand binding site. Binding of the IV.3 antibody can be blocked by clone FLI8.26 in flow cytometry analyses, suggesting that these clones may share a common or overlapping epitope.<\/p>\n

This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Human T Cell Enrichment Kit (Catalog #19051) and EasySep\u2122 Human CD4+ T Cell Enrichment Kit.<\/p><\/div>\n<\/div>\n<\/div>\n

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Subtype: Primary Antibodies<\/div>\n<\/div>\n
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Target Antigen: CD32<\/div>\n<\/div>\n
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Alternative Names: FCR II, Fc\u03b3RII<\/div>\n<\/div>\n
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Reactive Species: Human<\/div>\n<\/div>\n
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Conjugation: FITC; Unconjugated<\/div>\n<\/div>\n
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Host Species: Mouse<\/div>\n<\/div>\n
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Cell Type: B Cells; Granulocytes and Subsets; Monocytes<\/div>\n<\/div>\n
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Application: Cell Isolation; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunohistochemistry; Neutralization and Blocking; Western Blotting<\/div>\n<\/div>\n
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Area of Interest: Immunology<\/div>\n<\/div>\n
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Clone: IV.3<\/div>\n<\/div>\n
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Gene ID: 2212<\/div>\n<\/div>\n
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Isotype: IgG2b, kappa<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Overview The IV.3 antibody reacts with human CD32 (Fc\u03b3RII), an ~40 kDa type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, immunomodulation and platelet aggregation. CD32 is encoded by three genes (A, B, C) and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. All isoforms are expressed by monocytes\/macrophages, placental trophoblasts and endothelial cells. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. Isoform IIc is expressed by NK cells and neutrophils. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes. These interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the IV.3 antibody may be employed as a blocking antibody to reduce non-specific binding. The IV.3 antibody binds most strongly to the IIa isoforms of CD32, with the epitope mapped to amino acids 132 – 137 [FSHLDP] in domain 2, within the ligand binding site. Binding of the IV.3 antibody can be blocked by clone FLI8.26 in flow cytometry analyses, suggesting that these clones may share a common or overlapping epitope. This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Human T Cell Enrichment Kit (Catalog #19051) and EasySep\u2122 Human CD4+ T Cell Enrichment Kit. Subtype: Primary Antibodies Target Antigen: CD32 Alternative Names: FCR II, Fc\u03b3RII Reactive Species: Human Conjugation: FITC; Unconjugated Host Species: Mouse Cell Type: B Cells; Granulocytes and Subsets; Monocytes Application: Cell Isolation; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunohistochemistry; Neutralization and Blocking; Western Blotting Area of Interest: Immunology Clone: IV.3 Gene ID: 2212 Isotype: IgG2b, kappa<\/p>\n","protected":false},"featured_media":2561,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[138],"product_tag":[],"class_list":["post-2559","product","type-product","status-publish","has-post-thumbnail","product_cat-antibodies","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2559","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2561"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2559"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2559"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2559"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2559"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}