{"id":2580,"date":"2020-11-15T13:39:43","date_gmt":"2020-11-15T13:39:43","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2580"},"modified":"2020-11-15T13:39:43","modified_gmt":"2020-11-15T13:39:43","slug":"anti-mouse-mhc-class-ii-i-a-i-e-antibody-clone-m5-114-15-2-rat-monoclonal-igg2b-antibody-against-mouse-mhc-class-ii-i-a-i-e","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/anti-mouse-mhc-class-ii-i-a-i-e-antibody-clone-m5-114-15-2-rat-monoclonal-igg2b-antibody-against-mouse-mhc-class-ii-i-a-i-e\/","title":{"rendered":"Anti-Mouse MHC Class II (I-A\/I-E) Antibody, Clone M5\/114.15.2 Rat monoclonal IgG2b antibody against mouse MHC Class II (I-A\/I-E)"},"content":{"rendered":"

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Overview<\/h3>\n
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The M5\/114.15.2 (M5\/114) antibody reacts with an extracellular epitope on the mouse major histocompatibility complex class II receptor (MHC-II). MHC-II is a heterodimeric transmembrane glycoprotein comprising an \u03b1 subunit associated non-covalently with a \u03b2 subunit. In mice, MHC-II complexes are designated I-A and I-E and comprise A\u03b1\/A\u03b2 and E\u03b1\/E\u03b2 heterodimers, respectively. Together with the CD3\/T cell receptor (TCR) complex and CD4 molecules, MHC-II mediates a critical function in presenting peptides generated from hydrolysis of exogenous antigens by antigen-presenting cells to CD4+ T (helper) cells, thereby either suppressing or inducing an immune response to the peptides. The M5\/114.15.2 antibody recognizes a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek (but not I-Af, I-Ak, or I-As) MHC class II alloantigens expressed on B cells, dendritic cells, macrophages, monocytes, and activated T cells of mice carrying the H-2b, d, p, q, r, and u haplotypes (but not haplotypes H-2f or H-2s). The M5\/114 antibody reportedly inhibits I-A-restricted responses by T cells of the H-2b, d, q, and u (but not H-2f, k, or s) haplotypes. It does not react with cells from NOD (H-2g7) mice.<\/div>\n<\/div>\n<\/div>\n
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Subtype: Primary Antibodies<\/div>\n<\/div>\n
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Target Antigen: MHC Class II (I-A\/I-E)<\/div>\n<\/div>\n
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Alternative Names: IA, I-A, IA\/IE, I-A\/E, I-E, MHC class II, MHC-II<\/div>\n<\/div>\n
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Reactive Species: Mouse<\/div>\n<\/div>\n
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Conjugation: APC; FITC; PE; Unconjugated<\/div>\n<\/div>\n
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Host Species: Rat<\/div>\n<\/div>\n
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Cell Type: B Cells; Macrophages; Monocytes; T Cells<\/div>\n<\/div>\n
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Application: CyTOF; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Radioimmunoassay; Western Blotting<\/div>\n<\/div>\n
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Area of Interest: Immunology<\/div>\n<\/div>\n
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Clone: M5\/114.15.2<\/div>\n<\/div>\n
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Gene ID: 14961\/14969<\/div>\n<\/div>\n
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Isotype: IgG2b, kappa<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

  Overview The M5\/114.15.2 (M5\/114) antibody reacts with an extracellular epitope on the mouse major histocompatibility complex class II receptor (MHC-II). MHC-II is a heterodimeric transmembrane glycoprotein comprising an \u03b1 subunit associated non-covalently with a \u03b2 subunit. In mice, MHC-II complexes are designated I-A and I-E and comprise A\u03b1\/A\u03b2 and E\u03b1\/E\u03b2 heterodimers, respectively. Together with the CD3\/T cell receptor (TCR) complex and CD4 molecules, MHC-II mediates a critical function in presenting peptides generated from hydrolysis of exogenous antigens by antigen-presenting cells to CD4+ T (helper) cells, thereby either suppressing or inducing an immune response to the peptides. The M5\/114.15.2 antibody recognizes a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek (but not I-Af, I-Ak, or I-As) MHC class II alloantigens expressed on B cells, dendritic cells, macrophages, monocytes, and activated T cells of mice carrying the H-2b, d, p, q, r, and u haplotypes (but not haplotypes H-2f or H-2s). The M5\/114 antibody reportedly inhibits I-A-restricted responses by T cells of the H-2b, d, q, and u (but not H-2f, k, or s) haplotypes. It does not react with cells from NOD (H-2g7) mice. Subtype: Primary Antibodies Target Antigen: MHC Class II (I-A\/I-E) Alternative Names: IA, I-A, IA\/IE, I-A\/E, I-E, MHC class II, MHC-II Reactive Species: Mouse Conjugation: APC; FITC; PE; Unconjugated Host Species: Rat Cell Type: B Cells; Macrophages; Monocytes; T Cells Application: CyTOF; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Radioimmunoassay; Western Blotting Area of Interest: Immunology Clone: M5\/114.15.2 Gene ID: 14961\/14969 Isotype: IgG2b, kappa<\/p>\n","protected":false},"featured_media":2574,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[138],"product_tag":[],"class_list":["post-2580","product","type-product","status-publish","has-post-thumbnail","product_cat-antibodies","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2580","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2574"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2580"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2580"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2580"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2580"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}