{"id":2596,"date":"2020-11-16T10:35:52","date_gmt":"2020-11-16T10:35:52","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2596"},"modified":"2020-11-16T10:35:52","modified_gmt":"2020-11-16T10:35:52","slug":"anti-human-cd45-antibody-clone-hi30-mouse-monoclonal-igg1-antibody-against-human-chimpanzee-cd45","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/anti-human-cd45-antibody-clone-hi30-mouse-monoclonal-igg1-antibody-against-human-chimpanzee-cd45\/","title":{"rendered":"Anti-Human CD45 Antibody, Clone HI30 Mouse monoclonal IgG1 antibody against human, chimpanzee CD45"},"content":{"rendered":"

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Overview<\/h3>\n
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The HI30 antibody reacts with all isoforms of CD45, a type I transmembrane glycoprotein expressed on the surface of most hematopoietic cells except mature erythrocytes, platelets, and plasma cells; expression of CD45 is lost during differentiation of these cell types. CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including cell activation, growth, differentiation, and oncogenic transformation. The large cytoplasmic portion of CD45 contains two tyrosine phosphatase domains (one of which is enzymatically active) that are involved in modulating the function of intracellular substrates such as the Src kinases Lck and Fyn. Several isoforms of CD45 have been identified, the expression of which differs according to cell type and functional status. Alternative splicing of three exons (4, 5, 6) encoding the extracellular RA, RB, and RC polypeptide sequences gives rise to up to 8 isoforms with molecular masses in the range of 180 – 240 kDa. The leukocyte common antigen (LCA), the region recognized by the HI30 antibody, is an extracellular region located proximal to the membrane and common to all isoforms of CD45.<\/p>\n

This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Human T Cell Enrichment Kit (Catalog #19051), EasySep\u2122 Human CD4+ T Cell Enrichment Kit (Catalog #19052) and EasySep\u2122 Human CD45 Depletion Kit (Catalog #18259; partial blocking may be observed).<\/p><\/div>\n<\/div>\n<\/div>\n

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Subtype: Primary Antibodies<\/div>\n<\/div>\n
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Target Antigen: CD45<\/div>\n<\/div>\n
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Alternative Names: B220, LCA, T200<\/div>\n<\/div>\n
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Reactive Species: Chimpanzee; Human<\/div>\n<\/div>\n
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Conjugation: APC; FITC<\/div>\n<\/div>\n
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Host Species: Mouse<\/div>\n<\/div>\n
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Cell Type: Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells; Leukocytes; Lymphocytes; Myeloid Cells<\/div>\n<\/div>\n
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Application: Cell Isolation; CyTOF; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation<\/div>\n<\/div>\n
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Area of Interest: Immunology<\/div>\n<\/div>\n
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Clone: HI30<\/div>\n<\/div>\n
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Gene ID: 5788<\/div>\n<\/div>\n
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Isotype: IgG1, kappa<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Overview The HI30 antibody reacts with all isoforms of CD45, a type I transmembrane glycoprotein expressed on the surface of most hematopoietic cells except mature erythrocytes, platelets, and plasma cells; expression of CD45 is lost during differentiation of these cell types. CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including cell activation, growth, differentiation, and oncogenic transformation. The large cytoplasmic portion of CD45 contains two tyrosine phosphatase domains (one of which is enzymatically active) that are involved in modulating the function of intracellular substrates such as the Src kinases Lck and Fyn. Several isoforms of CD45 have been identified, the expression of which differs according to cell type and functional status. Alternative splicing of three exons (4, 5, 6) encoding the extracellular RA, RB, and RC polypeptide sequences gives rise to up to 8 isoforms with molecular masses in the range of 180 – 240 kDa. The leukocyte common antigen (LCA), the region recognized by the HI30 antibody, is an extracellular region located proximal to the membrane and common to all isoforms of CD45. This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Human T Cell Enrichment Kit (Catalog #19051), EasySep\u2122 Human CD4+ T Cell Enrichment Kit (Catalog #19052) and EasySep\u2122 Human CD45 Depletion Kit (Catalog #18259; partial blocking may be observed). Subtype: Primary Antibodies Target Antigen: CD45 Alternative Names: B220, LCA, T200 Reactive Species: Chimpanzee; Human Conjugation: APC; FITC Host Species: Mouse Cell Type: Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells; Leukocytes; Lymphocytes; Myeloid Cells Application: Cell Isolation; CyTOF; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation Area of Interest: Immunology Clone: HI30 Gene ID: 5788 Isotype: IgG1, kappa<\/p>\n","protected":false},"featured_media":2573,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[138],"product_tag":[],"class_list":["post-2596","product","type-product","status-publish","has-post-thumbnail","product_cat-antibodies","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2596","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2573"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2596"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2596"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2596"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2596"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}