{"id":2622,"date":"2020-11-17T14:09:01","date_gmt":"2020-11-17T14:09:01","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2622"},"modified":"2020-11-17T14:09:01","modified_gmt":"2020-11-17T14:09:01","slug":"anti-human-cd16-antibody-clone-3g8-mouse-monoclonal-igg1-antibody-against-human-rhesus-cynomolgus-cd16","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/anti-human-cd16-antibody-clone-3g8-mouse-monoclonal-igg1-antibody-against-human-rhesus-cynomolgus-cd16\/","title":{"rendered":"Anti-Human CD16 Antibody, Clone 3G8 Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD16"},"content":{"rendered":"

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Overview<\/h3>\n
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The 3G8 antibody reacts with CD16 (Fc\u03b3RIII or low affinity IgG receptor III), a type 1 transmembrane glycoprotein belonging to the Ig superfamily. CD16 exists in two distinct isoforms: CD16a, a 50 – 65 kDa form expressed on NK cells, activated monocytes, macrophages, and placental trophoblasts, and CD16b, an ~48 kDa glycosylphosphatidylinositol (GPI)-anchored form expressed on neutrophils, basophils and eosinophils and found as at least two polymorphic variants, termed NA1 and NA2. CD16 binds weakly to the Fc region of monomeric, aggregated or complexed IgG, particularly the IgG1 and IgG3 isotypes. Binding of IgG to either CD16 isoform induces signaling pathways that modulate several types of responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release and proliferation. CD16\/IgG interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the 3G8 antibody may be employed as a blocking antibody to reduce non-specific binding.<\/div>\n<\/div>\n<\/div>\n
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Subtype: Primary Antibodies<\/div>\n<\/div>\n
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Target Antigen: CD16<\/div>\n<\/div>\n
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Alternative Names: CD16A, CD16B, Fc-gamma RIII; FCG3; FCGR3; FCGRIII; Fc\u03b3RIII; FCR-10, FcRIII, IGFR3, IMD20<\/div>\n<\/div>\n
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Reactive Species: Baboon; Capuchin Monkey; Common Marmoset; Cotton-Topped Tamarin; Cynomolgus; Rhesus; Sooty Mangabey; Squirrel Monkey; Chimpanzee; Human; Macaque<\/div>\n<\/div>\n
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Conjugation: Alexa Fluor 488; APC; Biotin; FITC; PE; Unconjugated<\/div>\n<\/div>\n
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Host Species: Mouse<\/div>\n<\/div>\n
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Cell Type: Granulocytes and Subsets; Monocytes<\/div>\n<\/div>\n
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Application: Cell Isolation; CyTOF; Depletion; ELISA; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Neutralization and Blocking<\/div>\n<\/div>\n
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Area of Interest: Immunology<\/div>\n<\/div>\n
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Clone: 3G8<\/div>\n<\/div>\n
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Gene ID: 2214<\/div>\n<\/div>\n
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Isotype: IgG1, kappa<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

  Overview The 3G8 antibody reacts with CD16 (Fc\u03b3RIII or low affinity IgG receptor III), a type 1 transmembrane glycoprotein belonging to the Ig superfamily. CD16 exists in two distinct isoforms: CD16a, a 50 – 65 kDa form expressed on NK cells, activated monocytes, macrophages, and placental trophoblasts, and CD16b, an ~48 kDa glycosylphosphatidylinositol (GPI)-anchored form expressed on neutrophils, basophils and eosinophils and found as at least two polymorphic variants, termed NA1 and NA2. CD16 binds weakly to the Fc region of monomeric, aggregated or complexed IgG, particularly the IgG1 and IgG3 isotypes. Binding of IgG to either CD16 isoform induces signaling pathways that modulate several types of responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release and proliferation. CD16\/IgG interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the 3G8 antibody may be employed as a blocking antibody to reduce non-specific binding. Subtype: Primary Antibodies Target Antigen: CD16 Alternative Names: CD16A, CD16B, Fc-gamma RIII; FCG3; FCGR3; FCGRIII; Fc\u03b3RIII; FCR-10, FcRIII, IGFR3, IMD20 Reactive Species: Baboon; Capuchin Monkey; Common Marmoset; Cotton-Topped Tamarin; Cynomolgus; Rhesus; Sooty Mangabey; Squirrel Monkey; Chimpanzee; Human; Macaque Conjugation: Alexa Fluor 488; APC; Biotin; FITC; PE; Unconjugated Host Species: Mouse Cell Type: Granulocytes and Subsets; Monocytes Application: Cell Isolation; CyTOF; Depletion; ELISA; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Neutralization and Blocking Area of Interest: Immunology Clone: 3G8 Gene ID: 2214 Isotype: IgG1, kappa<\/p>\n","protected":false},"featured_media":2579,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[138],"product_tag":[],"class_list":["post-2622","product","type-product","status-publish","has-post-thumbnail","product_cat-antibodies","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2622","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2579"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2622"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2622"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2622"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2622"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}