{"id":2635,"date":"2020-11-18T10:11:51","date_gmt":"2020-11-18T10:11:51","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2635"},"modified":"2020-11-18T10:11:51","modified_gmt":"2020-11-18T10:11:51","slug":"anti-human-cd32-antibody-clone-fli8-26-mouse-monoclonal-igg2b-antibody-against-human-rhesus-cynomolgus-cd32","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/anti-human-cd32-antibody-clone-fli8-26-mouse-monoclonal-igg2b-antibody-against-human-rhesus-cynomolgus-cd32\/","title":{"rendered":"Anti-Human CD32 Antibody, Clone FLI8.26 Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD32"},"content":{"rendered":"

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Overview<\/h3>\n
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The FLI8.26 antibody reacts with human CD32 (Fc\u03b3RII), an ~40 kDa type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, immunomodulation, and platelet aggregation. The FLI8.26 antibody cross-reacts with monocytes, granulocytes, and platelets. CD32 is encoded by three genes (A, B, C) and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. All isoforms are expressed by monocytes\/macrophages, placental trophoblasts, and endothelial cells. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. Isoform IIc is expressed by NK cells and neutrophils. Clone FLI8.26 has been verified to bind to Fc\u03b3RIIa and Fc\u03b3RIIb, and reportedly binds to Fc\u03b3RIIc, which shares an identical amino acid sequence to Fc\u03b3RIIb in the signal peptide, extracellular and transmembrane domains. Clone FLI8.26 inhibits binding of the Fc\u03b3RIIa-specific clone IV.3 antibody in flow cytometry analyses. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes. These interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the FLI8.26 antibody may be used as a blocking antibody to reduce non-specific binding.<\/div>\n<\/div>\n<\/div>\n

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Subtype: Primary Antibodies<\/div>\n<\/div>\n
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Target Antigen: CD32<\/div>\n<\/div>\n
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Alternative Names: FCR II, Fc\u03b3RII<\/div>\n<\/div>\n
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Reactive Species: Human; Rhesus; Cynomolgus; Baboon; Chimpanzee<\/div>\n<\/div>\n
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Conjugation: Unconjugated<\/div>\n<\/div>\n
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Host Species: Mouse<\/div>\n<\/div>\n
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Cell Type: Monocytes; Granulocytes and Subsets; B Cells<\/div>\n<\/div>\n
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Application: Cell Isolation; Flow Cytometry<\/div>\n<\/div>\n
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Area of Interest: Immunology<\/div>\n<\/div>\n
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Clone: FLI8.26<\/div>\n<\/div>\n
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Gene ID: 2212<\/div>\n<\/div>\n
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Isotype: IgG2b, kappa<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Overview The FLI8.26 antibody reacts with human CD32 (Fc\u03b3RII), an ~40 kDa type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, immunomodulation, and platelet aggregation. The FLI8.26 antibody cross-reacts with monocytes, granulocytes, and platelets. CD32 is encoded by three genes (A, B, C) and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. All isoforms are expressed by monocytes\/macrophages, placental trophoblasts, and endothelial cells. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. Isoform IIc is expressed by NK cells and neutrophils. Clone FLI8.26 has been verified to bind to Fc\u03b3RIIa and Fc\u03b3RIIb, and reportedly binds to Fc\u03b3RIIc, which shares an identical amino acid sequence to Fc\u03b3RIIb in the signal peptide, extracellular and transmembrane domains. Clone FLI8.26 inhibits binding of the Fc\u03b3RIIa-specific clone IV.3 antibody in flow cytometry analyses. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes. These interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the FLI8.26 antibody may be used as a blocking antibody to reduce non-specific binding.     Subtype: Primary Antibodies Target Antigen: CD32 Alternative Names: FCR II, Fc\u03b3RII Reactive Species: Human; Rhesus; Cynomolgus; Baboon; Chimpanzee Conjugation: Unconjugated Host Species: Mouse Cell Type: Monocytes; Granulocytes and Subsets; B Cells Application: Cell Isolation; Flow Cytometry Area of Interest: Immunology Clone: FLI8.26 Gene ID: 2212 Isotype: IgG2b, kappa<\/p>\n","protected":false},"featured_media":2577,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[138],"product_tag":[],"class_list":["post-2635","product","type-product","status-publish","has-post-thumbnail","product_cat-antibodies","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2635","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2577"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2635"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2635"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2635"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2635"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}