{"id":2649,"date":"2020-11-18T14:06:49","date_gmt":"2020-11-18T14:06:49","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2649"},"modified":"2020-11-18T14:06:49","modified_gmt":"2020-11-18T14:06:49","slug":"anti-human-cd4-antibody-clone-okt4-mouse-monoclonal-igg2b-antibody-against-human-rhesus-cynomolgus-cd4","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/anti-human-cd4-antibody-clone-okt4-mouse-monoclonal-igg2b-antibody-against-human-rhesus-cynomolgus-cd4\/","title":{"rendered":"Anti-Human CD4 Antibody, Clone OKT4 Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD4"},"content":{"rendered":"

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Overview<\/h3>\n
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The OKT4 antibody reacts with CD4, an ~59 kDa single-chain type 1 transmembrane glycoprotein and member of the immunoglobulin (Ig) superfamily; CD4 contains four extracellular Ig-like domains (D1 – D4). The epitope for OKT4 has been localized to the D3 domain of the protein, which has a structure resembling an Ig variable domain. CD4 is expressed at relatively high levels by most thymocytes and a subpopulation of T cells (T-helper cells), and at lower levels by peripheral blood monocytes and macrophages. CD4 binds to a non-polymorphic region of MHC II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition by enhancing the affinity of the association between the TCR and MHC II-antigen complex. CD4 also functions to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. Moreover, CD4 is a receptor for human immunodeficiency virus (HIV). Binding of the OKT4 antibody to CD4 does not block HIV binding.<\/p>\n

This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Direct Human CD4+ T Cell Isolation Kit , EasySep\u2122 Human CD4+ T Cell Enrichment Kit, EasySep\u2122 Human CD3 Positive Selection Kit II and EasySep\u2122 Human CD4 Positive Selection Kit II.<\/p><\/div>\n<\/div>\n<\/div>\n

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Subtype: Primary Antibodies<\/div>\n<\/div>\n
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Target Antigen: CD4<\/div>\n<\/div>\n
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Alternative Names: T4<\/div>\n<\/div>\n
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Reactive Species: Human; Rhesus; Cynomolgus; Chimpanzee; Sooty Mangabey<\/div>\n<\/div>\n
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Conjugation: Alexa Fluor 488; APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated<\/div>\n<\/div>\n
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Host Species: Mouse<\/div>\n<\/div>\n
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Cell Type: T Cells; T Cells, Regulatory; T Cells, CD4+<\/div>\n<\/div>\n
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Application: Flow Cytometry; Functional Assay; Immunofluorescence; Immunohistochemistry; Immunoprecipitation<\/div>\n<\/div>\n
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Area of Interest: Immunology; Transplantation Research<\/div>\n<\/div>\n
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Clone: OKT4<\/div>\n<\/div>\n
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Gene ID: 920<\/div>\n<\/div>\n
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Isotype: IgG2b, kappa<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Overview The OKT4 antibody reacts with CD4, an ~59 kDa single-chain type 1 transmembrane glycoprotein and member of the immunoglobulin (Ig) superfamily; CD4 contains four extracellular Ig-like domains (D1 – D4). The epitope for OKT4 has been localized to the D3 domain of the protein, which has a structure resembling an Ig variable domain. CD4 is expressed at relatively high levels by most thymocytes and a subpopulation of T cells (T-helper cells), and at lower levels by peripheral blood monocytes and macrophages. CD4 binds to a non-polymorphic region of MHC II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition by enhancing the affinity of the association between the TCR and MHC II-antigen complex. CD4 also functions to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. Moreover, CD4 is a receptor for human immunodeficiency virus (HIV). Binding of the OKT4 antibody to CD4 does not block HIV binding. This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Direct Human CD4+ T Cell Isolation Kit , EasySep\u2122 Human CD4+ T Cell Enrichment Kit, EasySep\u2122 Human CD3 Positive Selection Kit II and EasySep\u2122 Human CD4 Positive Selection Kit II. Subtype: Primary Antibodies Target Antigen: CD4 Alternative Names: T4 Reactive Species: Human; Rhesus; Cynomolgus; Chimpanzee; Sooty Mangabey Conjugation: Alexa Fluor 488; APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated Host Species: Mouse Cell Type: T Cells; T Cells, Regulatory; T Cells, CD4+ Application: Flow Cytometry; Functional Assay; Immunofluorescence; Immunohistochemistry; Immunoprecipitation Area of Interest: Immunology; Transplantation Research Clone: OKT4 Gene ID: 920 Isotype: IgG2b, kappa<\/p>\n","protected":false},"featured_media":2577,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[138],"product_tag":[],"class_list":["post-2649","product","type-product","status-publish","has-post-thumbnail","product_cat-antibodies","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2649","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2577"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2649"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2649"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2649"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2649"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}