{"id":2666,"date":"2020-11-19T09:33:42","date_gmt":"2020-11-19T09:33:42","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2666"},"modified":"2020-11-19T09:33:43","modified_gmt":"2020-11-19T09:33:43","slug":"anti-human-cd8a-antibody-clone-rpa-t8-mouse-monoclonal-igg1-antibody-against-human-rhesus-cynomolgus-cd8a","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/anti-human-cd8a-antibody-clone-rpa-t8-mouse-monoclonal-igg1-antibody-against-human-rhesus-cynomolgus-cd8a\/","title":{"rendered":"Anti-Human CD8a Antibody, Clone RPA-T8 Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD8a"},"content":{"rendered":"

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Overview<\/h3>\n
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The RPA-T8 antibody reacts with CD8a, a 32 – 34 kDa type I transmembrane glycoprotein which is a subunit of CD8. CD8 is a disulfide-bonded dimer, found either as a heterodimer of CD8a (\u03b1) and CD8b (\u03b2) subunits (i.e., \u03b1\u03b2) or a homodimer (\u03b1\u03b1). CD8 acts as a co-receptor to the T cell receptor (TCR) during T cell activation by binding MHC Class I molecules presented by an antigen-presenting cell. It functions to strengthen the association between the TCR and MHC I-antigen complex, and to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. The CD8a chain binds to the \u03b13 domain of class I MHC molecules. CD8 is expressed by a majority of thymocytes, a subset of mature peripheral blood T cells (T cytotoxic cells), a proportion of \u03b2 T cells, and at lower levels by NK cells (which predominantly express CD8a homodimers).<\/p>\n

This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Direct Human CD8+ T Cell Isolation Kit , EasySep\u2122 Human CD8+ T Cell Enrichment Kit , EasySep\u2122 Human CD8 Positive Selection Kit , EasySep\u2122 Human CD3 Positive Selection Kit , EasySep\u2122 HLA CD2 Positive Selection Kit (Catalog #18657HLA), and EasySep\u2122 HLA T Cell Enrichment Kit.<\/p><\/div>\n<\/div>\n<\/div>\n

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Subtype: Primary Antibodies<\/div>\n<\/div>\n
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Target Antigen: CD8a<\/div>\n<\/div>\n
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Alternative Names: Leu2, T8<\/div>\n<\/div>\n
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Reactive Species: Baboon; Cynomolgus; Rhesus; Sooty Mangabey; Chimpanzee; Human; Macaque<\/div>\n<\/div>\n
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Conjugation: APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated<\/div>\n<\/div>\n
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Host Species: Mouse<\/div>\n<\/div>\n
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Cell Type: T Cells; T Cells, CD8+<\/div>\n<\/div>\n
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Application: Cell Isolation; CyTOF; Flow Cytometry; Functional Assay; Immunohistochemistry<\/div>\n<\/div>\n
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Area of Interest: Immunology<\/div>\n<\/div>\n
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Clone: RPA-T8<\/div>\n<\/div>\n
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Gene ID: 925<\/div>\n<\/div>\n
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Isotype: IgG1, kappa<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Overview The RPA-T8 antibody reacts with CD8a, a 32 – 34 kDa type I transmembrane glycoprotein which is a subunit of CD8. CD8 is a disulfide-bonded dimer, found either as a heterodimer of CD8a (\u03b1) and CD8b (\u03b2) subunits (i.e., \u03b1\u03b2) or a homodimer (\u03b1\u03b1). CD8 acts as a co-receptor to the T cell receptor (TCR) during T cell activation by binding MHC Class I molecules presented by an antigen-presenting cell. It functions to strengthen the association between the TCR and MHC I-antigen complex, and to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. The CD8a chain binds to the \u03b13 domain of class I MHC molecules. CD8 is expressed by a majority of thymocytes, a subset of mature peripheral blood T cells (T cytotoxic cells), a proportion of \u03b2 T cells, and at lower levels by NK cells (which predominantly express CD8a homodimers). This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Direct Human CD8+ T Cell Isolation Kit , EasySep\u2122 Human CD8+ T Cell Enrichment Kit , EasySep\u2122 Human CD8 Positive Selection Kit , EasySep\u2122 Human CD3 Positive Selection Kit , EasySep\u2122 HLA CD2 Positive Selection Kit (Catalog #18657HLA), and EasySep\u2122 HLA T Cell Enrichment Kit. Subtype: Primary Antibodies Target Antigen: CD8a Alternative Names: Leu2, T8 Reactive Species: Baboon; Cynomolgus; Rhesus; Sooty Mangabey; Chimpanzee; Human; Macaque Conjugation: APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated Host Species: Mouse Cell Type: T Cells; T Cells, CD8+ Application: Cell Isolation; CyTOF; Flow Cytometry; Functional Assay; Immunohistochemistry Area of Interest: Immunology Clone: RPA-T8 Gene ID: 925 Isotype: IgG1, kappa<\/p>\n","protected":false},"featured_media":2577,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[138],"product_tag":[],"class_list":["post-2666","product","type-product","status-publish","has-post-thumbnail","product_cat-antibodies","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2666","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2577"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2666"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2666"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2666"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2666"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}