{"id":2682,"date":"2020-11-19T10:51:32","date_gmt":"2020-11-19T10:51:32","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2682"},"modified":"2020-11-19T10:51:33","modified_gmt":"2020-11-19T10:51:33","slug":"anti-mouse-cd117-antibody-clone-2b8-rat-monoclonal-igg2b-antibody-against-mouse-cd117-c-kit","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/anti-mouse-cd117-antibody-clone-2b8-rat-monoclonal-igg2b-antibody-against-mouse-cd117-c-kit\/","title":{"rendered":"Anti-Mouse CD117 Antibody, Clone 2B8 Rat monoclonal IgG2b antibody against mouse CD117 (c-Kit)"},"content":{"rendered":"

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Overview<\/h3>\n
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The 2B8 antibody reacts with CD117 (c-Kit), an ~145 kDa type 1 transmembrane receptor for c-Kit ligand (stem cell factor\/steel factor) that is broadly expressed on hematopoietic stem cells in bone marrow, including pluripotent and erythroid progenitor cells and B and T lymphocyte precursors, as well as on mast cells. CD117 belongs to the tyrosine kinase receptor family and possesses five immunoglobulin-like C2-type domains and a cytoplasmic protein kinase domain. Binding of c-Kit ligand to CD117 induces dimerization and autophosphorylation, which activates several intracellular signaling pathways critical for the proliferation and differentiation of hematopoietic stem cells. Signaling by CD117 is modulated by phosphatases and by rapid endocytosis and degradation of the receptor. The 2B8 antibody does not block binding of c-Kit ligand. Mutations in CD117 are associated with various types of tumors and the piebald trait, an autosomal dominant abnormality of pigmentation.<\/p>\n

This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Mouse Hematopoietic Progenitor Cell Isolation Kit.<\/p><\/div>\n<\/div>\n<\/div>\n

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Subtype: Primary Antibodies<\/div>\n<\/div>\n
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Target Antigen: CD117 (c-Kit)<\/div>\n<\/div>\n
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Alternative Names: c-KIT, cKIT, Stem cell factor receptor (SCFR)<\/div>\n<\/div>\n
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Reactive Species: Mouse<\/div>\n<\/div>\n
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Conjugation: Alexa Fluor 488; APC; Biotin; FITC; PE; Unconjugated<\/div>\n<\/div>\n
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Host Species: Rat<\/div>\n<\/div>\n
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Cell Type: Hematopoietic Stem and Progenitor Cells<\/div>\n<\/div>\n
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Application: Cell Isolation; Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation<\/div>\n<\/div>\n
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Area of Interest: Stem Cell Biology<\/div>\n<\/div>\n
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Clone: 2B8<\/div>\n<\/div>\n
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Gene ID: 16590<\/div>\n<\/div>\n
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Isotype: IgG2b, kappa<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

  Overview The 2B8 antibody reacts with CD117 (c-Kit), an ~145 kDa type 1 transmembrane receptor for c-Kit ligand (stem cell factor\/steel factor) that is broadly expressed on hematopoietic stem cells in bone marrow, including pluripotent and erythroid progenitor cells and B and T lymphocyte precursors, as well as on mast cells. CD117 belongs to the tyrosine kinase receptor family and possesses five immunoglobulin-like C2-type domains and a cytoplasmic protein kinase domain. Binding of c-Kit ligand to CD117 induces dimerization and autophosphorylation, which activates several intracellular signaling pathways critical for the proliferation and differentiation of hematopoietic stem cells. Signaling by CD117 is modulated by phosphatases and by rapid endocytosis and degradation of the receptor. The 2B8 antibody does not block binding of c-Kit ligand. Mutations in CD117 are associated with various types of tumors and the piebald trait, an autosomal dominant abnormality of pigmentation. This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Mouse Hematopoietic Progenitor Cell Isolation Kit. Subtype: Primary Antibodies Target Antigen: CD117 (c-Kit) Alternative Names: c-KIT, cKIT, Stem cell factor receptor (SCFR) Reactive Species: Mouse Conjugation: Alexa Fluor 488; APC; Biotin; FITC; PE; Unconjugated Host Species: Rat Cell Type: Hematopoietic Stem and Progenitor Cells Application: Cell Isolation; Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation Area of Interest: Stem Cell Biology Clone: 2B8 Gene ID: 16590 Isotype: IgG2b, kappa<\/p>\n","protected":false},"featured_media":2577,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[138],"product_tag":[],"class_list":["post-2682","product","type-product","status-publish","has-post-thumbnail","product_cat-antibodies","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2682","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2577"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2682"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2682"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2682"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2682"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}