{"id":2740,"date":"2020-11-30T10:35:52","date_gmt":"2020-11-30T10:35:52","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2740"},"modified":"2020-11-30T10:35:52","modified_gmt":"2020-11-30T10:35:52","slug":"anti-mouse-cd25-antibody-clone-pc61-5-rat-monoclonal-igg1-antibody-against-mouse-cd25","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/anti-mouse-cd25-antibody-clone-pc61-5-rat-monoclonal-igg1-antibody-against-mouse-cd25\/","title":{"rendered":"Anti-Mouse CD25 Antibody, Clone PC61.5 Rat monoclonal IgG1 antibody against mouse CD25"},"content":{"rendered":"

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Overview<\/h3>\n
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The PC61.5 antibody reacts with mouse CD25 (low-affinity interleukin\u20102 receptor \u03b1 chain or IL-2R\u03b1), which is an ~55 kDa type 1 transmembrane glycoprotein expressed on T and B cell progenitors, activated (but not resting) T and B cells, regulatory T (Treg) cells, dendritic cells, and activated monocytes and macrophages. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells. CD25 per se has low affinity for its IL-2 ligand but associates with CD122 (IL-2R\u03b2) and CD132 (IL-2R\u03b3) to form the high-affinity IL-2R receptor. CD25 is necessary for receptor clustering and induction of signaling pathways involved in the activation and proliferation of lymphocytes. Binding of the PC61.5 antibody inhibits binding of IL-2 to the low- and high-affinity receptors, which may result in inhibition of IL-2-dependent proliferation. The epitope recognized by PC61.5 is distinct from the IL-2 binding site and from the epitopes recognized by antibodies 7D4 and 3C7.<\/div>\n<\/div>\n<\/div>\n
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Subtype: Primary Antibodies<\/div>\n<\/div>\n
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Target Antigen: CD25<\/div>\n<\/div>\n
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Alternative Names: IL-2 receptor subunit alpha, IL-2Ra, IL-2Ralpha, IL-2-RA, Interleukin-2 receptor subunit alpha, Ly-43, p55, Tac<\/div>\n<\/div>\n
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Reactive Species: Mouse<\/div>\n<\/div>\n
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Conjugation: APC; PE; Biotin; PerCP-Cyanine5.5; Unconjugated<\/div>\n<\/div>\n
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Host Species: Rat<\/div>\n<\/div>\n
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Cell Type: T Cells; T Cells, Regulatory<\/div>\n<\/div>\n
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Application: Flow Cytometry; Functional Assay; Immunohistochemistry; Immunoprecipitation; Depletion; Neutralization and Blocking<\/div>\n<\/div>\n
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Area of Interest: Immunology<\/div>\n<\/div>\n
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Clone: PC61.5<\/div>\n<\/div>\n
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Gene ID: 16184<\/div>\n<\/div>\n
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Isotype: IgG1, lambda<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

  Overview The PC61.5 antibody reacts with mouse CD25 (low-affinity interleukin\u20102 receptor \u03b1 chain or IL-2R\u03b1), which is an ~55 kDa type 1 transmembrane glycoprotein expressed on T and B cell progenitors, activated (but not resting) T and B cells, regulatory T (Treg) cells, dendritic cells, and activated monocytes and macrophages. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells. CD25 per se has low affinity for its IL-2 ligand but associates with CD122 (IL-2R\u03b2) and CD132 (IL-2R\u03b3) to form the high-affinity IL-2R receptor. CD25 is necessary for receptor clustering and induction of signaling pathways involved in the activation and proliferation of lymphocytes. Binding of the PC61.5 antibody inhibits binding of IL-2 to the low- and high-affinity receptors, which may result in inhibition of IL-2-dependent proliferation. The epitope recognized by PC61.5 is distinct from the IL-2 binding site and from the epitopes recognized by antibodies 7D4 and 3C7. Subtype: Primary Antibodies Target Antigen: CD25 Alternative Names: IL-2 receptor subunit alpha, IL-2Ra, IL-2Ralpha, IL-2-RA, Interleukin-2 receptor subunit alpha, Ly-43, p55, Tac Reactive Species: Mouse Conjugation: APC; PE; Biotin; PerCP-Cyanine5.5; Unconjugated Host Species: Rat Cell Type: T Cells; T Cells, Regulatory Application: Flow Cytometry; Functional Assay; Immunohistochemistry; Immunoprecipitation; Depletion; Neutralization and Blocking Area of Interest: Immunology Clone: PC61.5 Gene ID: 16184 Isotype: IgG1, lambda<\/p>\n","protected":false},"featured_media":2577,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[138],"product_tag":[],"class_list":["post-2740","product","type-product","status-publish","has-post-thumbnail","product_cat-antibodies","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2740","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2577"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2740"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2740"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2740"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2740"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}