{"id":2741,"date":"2020-11-30T10:37:33","date_gmt":"2020-11-30T10:37:33","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2741"},"modified":"2020-11-30T10:37:33","modified_gmt":"2020-11-30T10:37:33","slug":"anti-mouse-cd25-antibody-clone-3c7-rat-monoclonal-igg2b-antibody-against-mouse-cd25","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/anti-mouse-cd25-antibody-clone-3c7-rat-monoclonal-igg2b-antibody-against-mouse-cd25\/","title":{"rendered":"Anti-Mouse CD25 Antibody, Clone 3C7 Rat monoclonal IgG2b antibody against mouse CD25"},"content":{"rendered":"

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Overview<\/h3>\n
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The 3C7 antibody reacts with murine CD25 (interleukin\u20102 receptor \u03b1 chain; IL\u20102R\u03b1), an ~55 kDa type 1 transmembrane glycoprotein expressed on the surface of T and B cell progenitors and activated mature B and T cells. CD25 has been used as a marker to identify CD4+\/FoxP3+ regulatory T cells in mice. Mutations in CD25 result in severe immunodeficiency, underscoring the importance of the roles the protein plays in the differentiation, activation and proliferation of lymphocytes, and in the maintenance of self-tolerance. CD25 per se has low affinity for its IL-2 ligand but forms dimers with CD122 (IL-2R\u03b2) and CD132 (IL-2R\u03b3) that together associate to form the IL-2R receptor, which binds IL-2 with high affinity. CD25 acts to increase the specificity and affinity of IL-2 binding and is necessary for receptor clustering and signal transduction by the complex. Binding of the 3C7 antibody inhibits binding of IL\u20102 to free CD25 and the IL-2R receptor. The epitope is distinct from those recognized by the anti-CD25 antibody clones 7D4 and PC61.<\/p>\n

This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Mouse T Cell Enrichment Kit.<\/p><\/div>\n<\/div>\n<\/div>\n

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Subtype: Primary Antibodies<\/div>\n<\/div>\n
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Target Antigen: CD25<\/div>\n<\/div>\n
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Alternative Names: IL-2Ralpha, Ly-43, p55, Tac<\/div>\n<\/div>\n
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Reactive Species: Mouse<\/div>\n<\/div>\n
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Conjugation: FITC; PE; Unconjugated<\/div>\n<\/div>\n
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Host Species: Rat<\/div>\n<\/div>\n
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Cell Type: T Cells; T Cells, Regulatory<\/div>\n<\/div>\n
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Application: Cell Isolation; CyTOF; Flow Cytometry; Functional Assay; Immunohistochemistry<\/div>\n<\/div>\n
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Area of Interest: Immunology<\/div>\n<\/div>\n
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Clone: 3C7<\/div>\n<\/div>\n
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Gene ID: 16184<\/div>\n<\/div>\n
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Isotype: IgG2b, kappa<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

  Overview The 3C7 antibody reacts with murine CD25 (interleukin\u20102 receptor \u03b1 chain; IL\u20102R\u03b1), an ~55 kDa type 1 transmembrane glycoprotein expressed on the surface of T and B cell progenitors and activated mature B and T cells. CD25 has been used as a marker to identify CD4+\/FoxP3+ regulatory T cells in mice. Mutations in CD25 result in severe immunodeficiency, underscoring the importance of the roles the protein plays in the differentiation, activation and proliferation of lymphocytes, and in the maintenance of self-tolerance. CD25 per se has low affinity for its IL-2 ligand but forms dimers with CD122 (IL-2R\u03b2) and CD132 (IL-2R\u03b3) that together associate to form the IL-2R receptor, which binds IL-2 with high affinity. CD25 acts to increase the specificity and affinity of IL-2 binding and is necessary for receptor clustering and signal transduction by the complex. Binding of the 3C7 antibody inhibits binding of IL\u20102 to free CD25 and the IL-2R receptor. The epitope is distinct from those recognized by the anti-CD25 antibody clones 7D4 and PC61. This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Mouse T Cell Enrichment Kit. Subtype: Primary Antibodies Target Antigen: CD25 Alternative Names: IL-2Ralpha, Ly-43, p55, Tac Reactive Species: Mouse Conjugation: FITC; PE; Unconjugated Host Species: Rat Cell Type: T Cells; T Cells, Regulatory Application: Cell Isolation; CyTOF; Flow Cytometry; Functional Assay; Immunohistochemistry Area of Interest: Immunology Clone: 3C7 Gene ID: 16184 Isotype: IgG2b, kappa<\/p>\n","protected":false},"featured_media":2577,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[138],"product_tag":[],"class_list":["post-2741","product","type-product","status-publish","has-post-thumbnail","product_cat-antibodies","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2741","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2577"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2741"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2741"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2741"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2741"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}