{"id":2742,"date":"2020-11-30T11:11:47","date_gmt":"2020-11-30T11:11:47","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2742"},"modified":"2020-11-30T11:11:47","modified_gmt":"2020-11-30T11:11:47","slug":"anti-mouse-cd3e-antibody-clone-145-2c11-hamster-armenian-monoclonal-igg1-antibody-against-mouse-cd3e","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/anti-mouse-cd3e-antibody-clone-145-2c11-hamster-armenian-monoclonal-igg1-antibody-against-mouse-cd3e\/","title":{"rendered":"Anti-Mouse CD3e Antibody, Clone 145-2C11 Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e"},"content":{"rendered":"

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Overview<\/h3>\n
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The 145-2C11 antibody reacts with the ~20 kDa CD3\u03b5 subunit of the mouse T cell receptor (TCR)\/CD3 complex, which is expressed on the surface of circulating mature T cells and NKT cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3. The CD3 complex, which is assembled from combinations of CD3\u03b3, \u03b4, \u03b5, \u03b7, and \u03b6 subunits, associates non-covalently with the TCR and is involved in transducing antigen recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR. Activation of T cells by the TCR involves the cytoplasmic tails of the CD3 subunits, which are structurally related type 1 transmembrane proteins and members of the immunoglobulin superfamily. Mutations in the CD3 subunits have been associated with various immunodeficiency disorders including severed combined immunodeficiency (SCID). The 145-2C11 antibody has been used for in vitro functional (blocking and activation) assays and has been reported to block binding by the clone 17A2 antibody. The 145-2C11 antibody is not recommended for use with formalin-fixed, paraffin-embedded sections.<\/p>\n

This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Mouse T Cell Isolation Kit\u00a0 and EasySep\u2122 Mouse CD90.2 Positive Selection Kit II<\/p><\/div>\n<\/div>\n<\/div>\n

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Subtype: Primary Antibodies<\/div>\n<\/div>\n
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Target Antigen: CD3e<\/div>\n<\/div>\n
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Alternative Names: CD3, CD3epsilon, T3<\/div>\n<\/div>\n
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Reactive Species: Mouse<\/div>\n<\/div>\n
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Conjugation: Alexa Fluor 488; APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated<\/div>\n<\/div>\n
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Host Species: Hamster<\/div>\n<\/div>\n
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Cell Type: T Cells<\/div>\n<\/div>\n
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Application: Cell Isolation; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blotting; ELISA<\/div>\n<\/div>\n
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Area of Interest: Immunology<\/div>\n<\/div>\n
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Clone: 145-2C11<\/div>\n<\/div>\n
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Gene ID: 12501<\/div>\n<\/div>\n
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Isotype: IgG1, kappa<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

  Overview The 145-2C11 antibody reacts with the ~20 kDa CD3\u03b5 subunit of the mouse T cell receptor (TCR)\/CD3 complex, which is expressed on the surface of circulating mature T cells and NKT cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3. The CD3 complex, which is assembled from combinations of CD3\u03b3, \u03b4, \u03b5, \u03b7, and \u03b6 subunits, associates non-covalently with the TCR and is involved in transducing antigen recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR. Activation of T cells by the TCR involves the cytoplasmic tails of the CD3 subunits, which are structurally related type 1 transmembrane proteins and members of the immunoglobulin superfamily. Mutations in the CD3 subunits have been associated with various immunodeficiency disorders including severed combined immunodeficiency (SCID). The 145-2C11 antibody has been used for in vitro functional (blocking and activation) assays and has been reported to block binding by the clone 17A2 antibody. The 145-2C11 antibody is not recommended for use with formalin-fixed, paraffin-embedded sections. This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Mouse T Cell Isolation Kit\u00a0 and EasySep\u2122 Mouse CD90.2 Positive Selection Kit II Subtype: Primary Antibodies Target Antigen: CD3e Alternative Names: CD3, CD3epsilon, T3 Reactive Species: Mouse Conjugation: Alexa Fluor 488; APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated Host Species: Hamster Cell Type: T Cells Application: Cell Isolation; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blotting; ELISA Area of Interest: Immunology Clone: 145-2C11 Gene ID: 12501 Isotype: IgG1, kappa<\/p>\n","protected":false},"featured_media":2577,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[138],"product_tag":[],"class_list":["post-2742","product","type-product","status-publish","has-post-thumbnail","product_cat-antibodies","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2742","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2577"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2742"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2742"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2742"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2742"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}