{"id":2768,"date":"2020-11-30T14:12:11","date_gmt":"2020-11-30T14:12:11","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2768"},"modified":"2020-11-30T14:12:11","modified_gmt":"2020-11-30T14:12:11","slug":"anti-mouse-sca1-antibody-clone-e13-161-7-rat-monoclonal-igg2a-antibody-against-mouse-sca1-ly-6a-e","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/anti-mouse-sca1-antibody-clone-e13-161-7-rat-monoclonal-igg2a-antibody-against-mouse-sca1-ly-6a-e\/","title":{"rendered":"Anti-Mouse Sca1 Antibody, Clone E13-161.7 Rat monoclonal IgG2a antibody against mouse Sca1 (Ly-6A\/E)"},"content":{"rendered":"

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Overview<\/h3>\n
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The E13-161.7 antibody reacts with Sca1 (Stem Cell Antigen-1 or Ly-6A\/E), an 18 kDa GPI-linked protein belonging to the lymphocyte activation protein-6 (Ly-6) family. Sca1 is expressed on the surface of hematopoietic stem and progenitor cells, myeloid cells, and peripheral B and T lymphocytes. Sca1 is expressed by mice with either the Ly-6.1 or Ly-6.2 allotypes but the pattern of expression differs in the circulating cell population according to the allotype. Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA\/2, PL, SJL, SWR, 129) possess relatively high numbers of Sca1+ resting lymphocytes compared to Ly-6.1 strains (e.g. A, BALB\/c, CBA, C3H\/He, DBA\/1, NZB). Sca1 expression levels are strongly upregulated in all strains upon cellular activation. Sca1 is involved in the regulation of T and B cell responses and is believed to play roles in the differentiation, proliferation, and survival of a variety of stem cells. Sca1 has emerged as a phenotypic marker of choice for identifying and isolating hematopoietic stem and progenitor cells.<\/p>\n

This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Mouse SCA1 Biotin Positive Selection Kit<\/p><\/div>\n<\/div>\n<\/div>\n

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Subtype: Primary Antibodies<\/div>\n<\/div>\n
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Target Antigen: Sca1 (Ly-6A\/E)<\/div>\n<\/div>\n
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Alternative Names: Ly-6A\/E, Sca-1<\/div>\n<\/div>\n
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Reactive Species: Mouse<\/div>\n<\/div>\n
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Conjugation: Alexa Fluor 488; APC; Biotin; FITC; PE; Unconjugated<\/div>\n<\/div>\n
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Host Species: Rat<\/div>\n<\/div>\n
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Cell Type: Hematopoietic Stem and Progenitor Cells; Mammary Cells<\/div>\n<\/div>\n
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Application: Cell Isolation; Functional Assay; Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation<\/div>\n<\/div>\n
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Area of Interest: Stem Cell Biology; Epithelial Cell Biology<\/div>\n<\/div>\n
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Clone: E13-161.7<\/div>\n<\/div>\n
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Gene ID: 110454<\/div>\n<\/div>\n
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Isotype: IgG2a, kappa<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Overview The E13-161.7 antibody reacts with Sca1 (Stem Cell Antigen-1 or Ly-6A\/E), an 18 kDa GPI-linked protein belonging to the lymphocyte activation protein-6 (Ly-6) family. Sca1 is expressed on the surface of hematopoietic stem and progenitor cells, myeloid cells, and peripheral B and T lymphocytes. Sca1 is expressed by mice with either the Ly-6.1 or Ly-6.2 allotypes but the pattern of expression differs in the circulating cell population according to the allotype. Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA\/2, PL, SJL, SWR, 129) possess relatively high numbers of Sca1+ resting lymphocytes compared to Ly-6.1 strains (e.g. A, BALB\/c, CBA, C3H\/He, DBA\/1, NZB). Sca1 expression levels are strongly upregulated in all strains upon cellular activation. Sca1 is involved in the regulation of T and B cell responses and is believed to play roles in the differentiation, proliferation, and survival of a variety of stem cells. Sca1 has emerged as a phenotypic marker of choice for identifying and isolating hematopoietic stem and progenitor cells. This antibody clone has been verified for purity assessments of cells isolated with EasySep\u2122 kits, including EasySep\u2122 Mouse SCA1 Biotin Positive Selection Kit Subtype: Primary Antibodies Target Antigen: Sca1 (Ly-6A\/E) Alternative Names: Ly-6A\/E, Sca-1 Reactive Species: Mouse Conjugation: Alexa Fluor 488; APC; Biotin; FITC; PE; Unconjugated Host Species: Rat Cell Type: Hematopoietic Stem and Progenitor Cells; Mammary Cells Application: Cell Isolation; Functional Assay; Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation Area of Interest: Stem Cell Biology; Epithelial Cell Biology Clone: E13-161.7 Gene ID: 110454 Isotype: IgG2a, kappa<\/p>\n","protected":false},"featured_media":2577,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[138],"product_tag":[],"class_list":["post-2768","product","type-product","status-publish","has-post-thumbnail","product_cat-antibodies","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2768","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2577"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2768"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2768"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2768"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2768"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}