{"id":2836,"date":"2020-12-03T08:55:30","date_gmt":"2020-12-03T08:55:30","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2836"},"modified":"2020-12-03T08:55:30","modified_gmt":"2020-12-03T08:55:30","slug":"aldefluor-kit-for-the-identification-evaluation-and-isolation-of-stem-and-progenitor-cells-expressing-high-levels-of-aldh","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/aldefluor-kit-for-the-identification-evaluation-and-isolation-of-stem-and-progenitor-cells-expressing-high-levels-of-aldh\/","title":{"rendered":"ALDEFLUOR\u2122 Kit For the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH"},"content":{"rendered":"

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Overview<\/h3>\n
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The ALDEFLUOR\u2122 reagent system is a non-immunological method to identify stem\/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity. High ALDH expression has been reported for normal and cancer precursor cells of various lineages, including hematopoietic, mammary, endothelial, mesenchymal, and neural. Cells expressing high levels of ALDH become brightly fluorescent (ALDHbr) and can be identified and enumerated using a standard flow cytometer or isolated by cell sorting for further purification and characterization.<\/p>\n

The use of an internal cellular enzyme for identifying and isolating stem and progenitor cells provides an alternative to the more traditional method of staining with antibodies against cell surface antigens.<\/p><\/div>\n<\/div>\n<\/div>\n

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Advantages:<\/div>\n
\u2022 Identify viable cells with elevated ALDH activity
\n\u2022 Can be used to identify and isolate normal and malignant stem and progenitor cells across a wide variety of tissues<\/div>\n<\/div>\n
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Components:<\/div>\n
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  • ALDEFLUOR\u2122 Kit (Catalog #01700)\n
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    • Dry ALDEFLUOR\u2122 reagent, 50 \u00b5g<\/li>\n
    • DEAB Reagent, 1.5 mM, in 95% ethanol, 1 mL (Catalog #01705)<\/li>\n
    • HCL, 1.5 mL<\/li>\n
    • DMSO, 1.5 mL<\/li>\n
    • ALDEFLUOR\u2122 Assay Buffer, 4 x 25 mL (Catalog #01701)<\/li>\n
    • ALDEFLUOR\u2122 Quick Reference Guide<\/li>\n<\/ul>\n<\/li>\n
    • ALDEFLUOR\u2122 DEAB Reagent, 1.5 mM, 1 mL (Catalog #01705)\n
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      • DEAB Reagent, 1.5 mM, in 95% ethanol, 1 mL (Catalog #01705)<\/li>\n
      • <\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
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        Application: Flow Cytometry<\/div>\n<\/div>\n
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        Brand: ALDEFLUOR<\/div>\n<\/div>\n
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        Area of Interest: Cancer Research; Epithelial Cell Biology; Neuroscience; Stem Cell Biology<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

        Overview The ALDEFLUOR\u2122 reagent system is a non-immunological method to identify stem\/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity. High ALDH expression has been reported for normal and cancer precursor cells of various lineages, including hematopoietic, mammary, endothelial, mesenchymal, and neural. Cells expressing high levels of ALDH become brightly fluorescent (ALDHbr) and can be identified and enumerated using a standard flow cytometer or isolated by cell sorting for further purification and characterization. The use of an internal cellular enzyme for identifying and isolating stem and progenitor cells provides an alternative to the more traditional method of staining with antibodies against cell surface antigens. Advantages: \u2022 Identify viable cells with elevated ALDH activity \u2022 Can be used to identify and isolate normal and malignant stem and progenitor cells across a wide variety of tissues Components: ALDEFLUOR\u2122 Kit (Catalog #01700) Dry ALDEFLUOR\u2122 reagent, 50 \u00b5g DEAB Reagent, 1.5 mM, in 95% ethanol, 1 mL (Catalog #01705) HCL, 1.5 mL DMSO, 1.5 mL ALDEFLUOR\u2122 Assay Buffer, 4 x 25 mL (Catalog #01701) ALDEFLUOR\u2122 Quick Reference Guide ALDEFLUOR\u2122 DEAB Reagent, 1.5 mM, 1 mL (Catalog #01705) DEAB Reagent, 1.5 mM, in 95% ethanol, 1 mL (Catalog #01705) Application: Flow Cytometry Brand: ALDEFLUOR Area of Interest: Cancer Research; Epithelial Cell Biology; Neuroscience; Stem Cell Biology<\/p>\n","protected":false},"featured_media":2837,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[141],"product_tag":[],"class_list":["post-2836","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-dyes-and-detection-assay-kits","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2836","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2837"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2836"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2836"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2836"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2836"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}