{"id":2839,"date":"2020-12-03T08:58:55","date_gmt":"2020-12-03T08:58:55","guid":{"rendered":"https:\/\/mira-lab.com\/?post_type=product&p=2839"},"modified":"2020-12-03T08:58:55","modified_gmt":"2020-12-03T08:58:55","slug":"aldhbr-assay-kit-for-the-detection-of-cd34-and-aldhbr-cells-in-human-cord-blood","status":"publish","type":"product","link":"https:\/\/mira-lab.com\/new\/product\/aldhbr-assay-kit-for-the-detection-of-cd34-and-aldhbr-cells-in-human-cord-blood\/","title":{"rendered":"ALDHbr Assay Kit For the detection of CD34+ and ALDHbr cells in human cord blood"},"content":{"rendered":"

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Overview<\/h3>\n
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The ALDHbr Assay Kit is optimized for the identification and quantitation of CD34+ cells that express high levels of the enzyme aldehyde dehydrogenase (ALDH) in human cord blood samples. This kit contains both the CD34+ Cell Detection Kit and the ALDEFLUOR\u2122 Kit. The CD34+ Cell Detection Kit contains pre-selected fluorochrome-conjugated monoclonal antibodies that have been optimized for use with the ALDEFLUOR\u2122 Kit to detect cells that express CD34 and have high ALDH activity by flow cytometry. The CD34+ Cell Detection Kit contains anti-human antibodies APC CD34, PE CD45, and PE-Cyanine5 Glycophorin A\/B, and 7-AAD viability dye.<\/p>\n

A flow cytometer equipped with a 488 nm laser and a 635 nm laser for excitation and appropriate filters for detecting APC, PE, PE-Cyanine5 and 7-AAD fluorescence is required for use.<\/p><\/div>\n<\/div>\n<\/div>\n

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Advantages:<\/div>\n
\u2022 Quantifies primitive HSCs in < 1 day
\n\u2022 Optimized to detect ALDHbrCD34+ cells in CB
\n\u2022 Enumerates viable, metabolically active, primitive cells in the CD34+ cell population<\/div>\n<\/div>\n
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Components:<\/div>\n
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ALDHbr Assay Kit (Catalog #01711)<\/div>\n
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    1 x ALDEFLUOR\u2122 Kit (Catalog #01700)<\/div>\n
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      ALDEFLUOR\u2122 Reagent<\/div>\n<\/li>\n
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      ALDEFLUOR\u2122 DEAB Reagent<\/div>\n<\/li>\n
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      ALDEFLUOR\u2122 Assay Buffer<\/div>\n<\/li>\n
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      2 N HCl<\/div>\n<\/li>\n
    • \n
      DMSO<\/div>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n
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        2 x CD34+ Cell Detection Kits<\/div>\n
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          7-AAD Viability Dye<\/div>\n<\/li>\n
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          Anti-Human CD45 Antibody, Clone HI30, PE<\/div>\n<\/li>\n
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          Anti-Human CD34 Antibody, Clone 581, APC<\/div>\n<\/li>\n
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          Anti-Human CD235ab (Glycophorin AB) Antibody, Clone HIR2, PE-Cyanine5<\/div>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
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          Application: Flow Cytometry<\/div>\n<\/div>\n
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          Area of Interest: Cord Blood Banking; Stem Cell Biology<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

          Overview The ALDHbr Assay Kit is optimized for the identification and quantitation of CD34+ cells that express high levels of the enzyme aldehyde dehydrogenase (ALDH) in human cord blood samples. This kit contains both the CD34+ Cell Detection Kit and the ALDEFLUOR\u2122 Kit. The CD34+ Cell Detection Kit contains pre-selected fluorochrome-conjugated monoclonal antibodies that have been optimized for use with the ALDEFLUOR\u2122 Kit to detect cells that express CD34 and have high ALDH activity by flow cytometry. The CD34+ Cell Detection Kit contains anti-human antibodies APC CD34, PE CD45, and PE-Cyanine5 Glycophorin A\/B, and 7-AAD viability dye. A flow cytometer equipped with a 488 nm laser and a 635 nm laser for excitation and appropriate filters for detecting APC, PE, PE-Cyanine5 and 7-AAD fluorescence is required for use. Advantages: \u2022 Quantifies primitive HSCs in < 1 day \u2022 Optimized to detect ALDHbrCD34+ cells in CB \u2022 Enumerates viable, metabolically active, primitive cells in the CD34+ cell population Components: ALDHbr Assay Kit (Catalog #01711) 1 x ALDEFLUOR\u2122 Kit (Catalog #01700) ALDEFLUOR\u2122 Reagent ALDEFLUOR\u2122 DEAB Reagent ALDEFLUOR\u2122 Assay Buffer 2 N HCl DMSO 2 x CD34+ Cell Detection Kits 7-AAD Viability Dye Anti-Human CD45 Antibody, Clone HI30, PE Anti-Human CD34 Antibody, Clone 581, APC Anti-Human CD235ab (Glycophorin AB) Antibody, Clone HIR2, PE-Cyanine5 Application: Flow Cytometry Area of Interest: Cord Blood Banking; Stem Cell Biology<\/p>\n","protected":false},"featured_media":2840,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[141],"product_tag":[],"class_list":["post-2839","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-dyes-and-detection-assay-kits","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/2839","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/2840"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=2839"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=2839"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=2839"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=2839"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}