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ANTITHROMBIN III (ATIII) is the main antagonist of thrombin at forming of this enzyme in blood. At absence of heparin ATIII slowly and
\nirreversibly inhibit thrombin and other coagulation and fibrinolysis factors. At presence of heparin ATIII becomes rapid inhibitor of these enzymes.
\nHereditary and innate deficit of ATIII in blood leads to development of disseminated intravascular clotting (DIC) and development of acute thrombosis.
\nReduced concentration of ATIII is observed among patients with liver disease (cirrhosis), with nephrotic syndrome in the result of excretion of inhibitor with
\nurea. Concentration of ATIII is reduced by oral contraceptives and heparin injection. Principle. Method of the detection of AT III activity
\nis based on possibility of AT III to neutralize thrombin in heparin presence. Activity of AT III is detected in plasma by adding thrombin surplus to
\nit. At this time inhibition of thrombin is occurred by complex AT III-heparin proportionally to quantity of AT III in plasma. Residuary quantity of thrombin
\ncatalyses detachment of dye para-nitroaniline (pNA) from synthetic chromogen substrate. Absorption of free pNA, which is detected at wave-length 405
\nnm, is inversely proportional to AT III activity. Process has following scheme:
\nAT III + heparin (surplus) ==> AT III \u2013 heparin
\nAT III \u2013 heparin + thrombin (surplus) ==>
\nAT III \u2013 heparin \u2013 thrombin + thrombin (residue).
\nSubstrate – pNA + thrombin (residue) ==> peptide
\n+ pNA<\/p>\n
REACHROM \u2013 AT III<\/strong> <\/p>\n","protected":false},"excerpt":{"rendered":" ANTITHROMBIN III (ATIII) is the main antagonist of thrombin at forming of this enzyme in blood. At absence of heparin ATIII slowly and irreversibly inhibit thrombin and other coagulation and fibrinolysis factors. At presence of heparin ATIII becomes rapid inhibitor of these enzymes. Hereditary and innate deficit of ATIII in blood leads to development of disseminated intravascular clotting (DIC) and development of acute thrombosis. Reduced concentration of ATIII is observed among patients with liver disease (cirrhosis), with nephrotic syndrome in the result of excretion of inhibitor with urea. Concentration of ATIII is reduced by oral contraceptives and heparin injection. Principle. Method of the detection of AT III activity is based on possibility of AT III to neutralize thrombin in heparin presence. Activity of AT III is detected in plasma by adding thrombin surplus to it. At this time inhibition of thrombin is occurred by complex AT III-heparin proportionally to quantity of AT III in plasma. Residuary quantity of thrombin catalyses detachment of dye para-nitroaniline (pNA) from synthetic chromogen substrate. Absorption of free pNA, which is detected at wave-length 405 nm, is inversely proportional to AT III activity. Process has following scheme: AT III + heparin (surplus) ==> AT III \u2013 heparin AT III \u2013 heparin + thrombin (surplus) ==> AT III \u2013 heparin \u2013 thrombin + thrombin (residue). Substrate – pNA + thrombin (residue) ==> peptide + pNA REACHROM \u2013 AT III The kit of reagents for the determination antithrombin III activity by photometric method Cat. # PFA-2 for 40 assays. Reagents: – buffer with heparin, concentrated (5 mL) \u2013 2 vials – human thrombin (2.5 mL), lyophilized (10 IU\/mL) \u2013 2 vials – standard plasma (1 mL), lyophilized \u2013 1 vial – chromogen substrate (2 mL), lyophilized \u2013 2 vials <\/p>\n","protected":false},"featured_media":4397,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[206],"product_tag":[],"class_list":["post-4396","product","type-product","status-publish","has-post-thumbnail","product_cat-renam","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/4396","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/4397"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=4396"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=4396"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=4396"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=4396"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nThe kit of reagents for the determination antithrombin III activity by<\/strong>
\nphotometric method<\/strong>
\nCat. # PFA-2 for 40 assays.
\nReagents:
\n– buffer with heparin, concentrated (5 mL) \u2013 2 vials
\n– human thrombin (2.5 mL), lyophilized (10 IU\/mL) \u2013 2 vials
\n– standard plasma (1 mL), lyophilized \u2013 1 vial
\n– chromogen substrate (2 mL), lyophilized \u2013 2 vials<\/p>\n