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Heparin, acid mucopolysaccharide, is produced by mast cells. It is referred to direct anticoagulants, i.e. influence directly on coagulation factors (XII, XI, X, IX, VII and REACHROM \u2013 HEPARIN<\/strong> <\/p>\n <\/p>\n <\/p>\n <\/p>\n REACLOT-HEPARIN<\/strong> <\/p>\n <\/p>\n <\/p>\n <\/p>\n <\/p>\n RENAPARIN<\/strong> Heparin, acid mucopolysaccharide, is produced by mast cells. It is referred to direct anticoagulants, i.e. influence directly on coagulation factors (XII, XI, X, IX, VII and II). Heparin is used as anticoagulant for therapeutic purposes. The quantitative determination of heparin levels in plasma is useful tool for monitoring treatment efficacy. This determination may be performed using the following anti-Xa methods: clotting assays and chromogenic assays. Antithrombin III (AT III) is the main inhibitor of thrombin. It activates factor Xa and other serine proteinases of coagulation cascade. In normal conditions inhibition speed is low, but it rises in thousands times in heparin presence. Heparin preparations in complex with AT III inhibit action of activated factor Xa in more level than thrombin. On this reason test for inhibition of factor Xa activity is more sensitive and informative of all methods of detection of heparin activity in blood plasma. Principle. Method of the detection of heparin activity is based on ability of complex AT III-heparin to neutralize FXa. Plasma Heparin activity is determined by adding antithrombin III and FXa. At this time inhibition of factor Xa by complex AT III\u2013heparin proportional to heparin quantity in plasma. Residue factor Xa catalyze detachment of pNA from synthetic chromogen substrate. Absorption of free pNA, detected at wave-length as 405 nm, is inversely proportional to heparin activity in plasma. Process has following scheme: AT III (surplus) + heparin ==> AT III \u2013 heparin AT III \u2013 heparin + Xa (surplus) ==> AT III \u2013 heparin \u2013 Xa + Xa (residue) Substrate \u2013 pNA + Xa (residue) ==> peptide + pNA REACHROM \u2013 HEPARIN The kit of reagents for the determination of anti-Xa heparin activity by photometric method Cat. # HP-1 for 20-100 assays. Reagents: – buffer with heparin, concentrated (5 mL) \u2013 2 vials – human thrombin (2.5 mL), lyophilized (10 IU\/mL) \u2013 2 vials – standard plasma (1 mL), lyophilized \u2013 1 vial – chromogen substrate (2 mL), lyophilized \u2013 2 vials REACLOT-HEPARIN The kit of reagents for the determination of anti-Xa heparin activity by clotting method Cat. # HP-2-for 40-80 assays. The kit is intended for monitoring for heparin administration (including low molecular heparin) by the detection of its Xa activity in human blood plasma. Method is based on ability of small quantities of heparin in tested plasma to neutralize exogenous activated factor Xa in antithrombin III presence. Process has two stages: 1. inactivation of factor Xa surplus by complex AT III \u2013 heparin 2. measurement of coagulometric activity of residue factor Xa on phospholipid membrane in presence of calcium ions. Substrate plasma is the source of AT III, fibrinogen and factor V. RENAPARIN The kit of reagents for determination anti-Xa and anti-IIa activity low molecular heparin in medicament and substace. Cat. # HP-6 for 100 assays.<\/p>\n","protected":false},"featured_media":4407,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[206],"product_tag":[],"class_list":["post-4405","product","type-product","status-publish","has-post-thumbnail","product_cat-renam","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/4405","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/4407"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=4405"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=4405"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=4405"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=4405"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nII). Heparin is used as anticoagulant for therapeutic purposes. The quantitative determination of heparin levels in plasma is useful tool for monitoring treatment efficacy.
\nThis determination may be performed using the following anti-Xa methods: clotting assays and chromogenic assays. Antithrombin III (AT III) is the main inhibitor
\nof thrombin. It activates factor Xa and other serine proteinases of coagulation cascade. In normal conditions inhibition speed is low, but it rises in thousands times
\nin heparin presence. Heparin preparations in complex with AT III inhibit action of activated factor Xa in more level than thrombin. On this reason test for inhibition of
\nfactor Xa activity is more sensitive and informative of all methods of detection of heparin activity in blood plasma.
\nPrinciple. Method of the detection of heparin activity is based on ability of complex AT III-heparin to neutralize
\nFXa. Plasma Heparin activity is determined by adding antithrombin III and FXa. At this time inhibition of
\nfactor Xa by complex AT III\u2013heparin proportional to heparin quantity in plasma. Residue factor Xa catalyze
\ndetachment of pNA from synthetic chromogen substrate. Absorption of free pNA, detected at wave-length as 405 nm, is inversely proportional to heparin activity in plasma.
\nProcess has following scheme:<\/strong>
\nAT III (surplus) + heparin ==> AT III \u2013 heparin
\nAT III \u2013 heparin + Xa (surplus) ==> AT III \u2013 heparin \u2013
\nXa + Xa (residue)
\nSubstrate \u2013 pNA + Xa (residue) ==> peptide + pNA<\/p>\n
\nThe kit of reagents for the determination of anti-Xa heparin<\/strong>
\nactivity by photometric method<\/strong>
\nCat. # HP-1 for 20-100 assays.
\nReagents:
\n– buffer with heparin, concentrated (5 mL) \u2013 2 vials
\n– human thrombin (2.5 mL), lyophilized (10 IU\/mL) \u2013 2 vials
\n– standard plasma (1 mL), lyophilized \u2013 1 vial
\n– chromogen substrate (2 mL), lyophilized \u2013 2 vials<\/p>\n
\nThe kit of reagents for the determination of anti-Xa heparin activity by clotting method
\nCat. # HP-2-for 40-80 assays.
\nThe kit is intended for monitoring for heparin administration (including low molecular heparin) by the detection of its Xa
\nactivity in human blood plasma.
\nMethod is based on ability of small quantities of heparin in tested plasma to neutralize exogenous activated factor Xa
\nin antithrombin III presence.
\nProcess has two stages:
\n1. inactivation of factor Xa surplus by complex AT III \u2013 heparin
\n2. measurement of coagulometric activity of residue factor Xa on
\nphospholipid membrane in presence of calcium ions.
\nSubstrate plasma is the source of AT III, fibrinogen and factor V.![\"\"](\"https:\/\/mira-lab.com\/wp-content\/uploads\/2022\/01\/Untitled-20.png\")
<\/p>\n
\nThe kit of reagents for determination anti-Xa and anti-IIa activity low molecular heparin in medicament and substace.
\nCat. # HP-6 for 100 assays.<\/p>\n<\/p>\n","protected":false},"excerpt":{"rendered":"