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2-antiplasmin is an inhibitor of fibrinolysis, very fast binding plasmin, and irreversibly inhibiting
\nit. It is a single-stranded glycoprotein having a molecular weight of 60-70 kDt and migrating during electrophoresis 2-globulin. Principle. Method of the determination of
\n2-antiplasmin activity is based on its ability to neutralize of plasmin. Activity 2-antiplasmin plasma is determined by adding to her excess
\nplasmin\u2019s. In this case, the inhibition of plasmin is proportional to the amount of 2-antiplasmin in the plasma. The remaining amount of
\nplasmin catalyzes the cleavage paranitroaniline (RNA) from a synthetic chromogenic substrate.
\nAbsorption of free pNA determined at 405 nm is inversely proportional to activity 2-antiplasmin in
\nthe plasma.
\nThe process is as follows:
\n2-antiplasmin (plasma)+ plasmin (excess) ==>
\n( 2-antiplasmin-plasmin) + plasmin (residue).
\nSubstrate – RNA + plasmin (residue) ==> Peptide
\n+ RNA<\/p>\n
REACHROM- 2 ANTIPLASMIN<\/strong> <\/p>\n","protected":false},"excerpt":{"rendered":" 2-antiplasmin is an inhibitor of fibrinolysis, very fast binding plasmin, and irreversibly inhibiting it. It is a single-stranded glycoprotein having a molecular weight of 60-70 kDt and migrating during electrophoresis 2-globulin. Principle. Method of the determination of 2-antiplasmin activity is based on its ability to neutralize of plasmin. Activity 2-antiplasmin plasma is determined by adding to her excess plasmin\u2019s. In this case, the inhibition of plasmin is proportional to the amount of 2-antiplasmin in the plasma. The remaining amount of plasmin catalyzes the cleavage paranitroaniline (RNA) from a synthetic chromogenic substrate. Absorption of free pNA determined at 405 nm is inversely proportional to activity 2-antiplasmin in the plasma. The process is as follows: 2-antiplasmin (plasma)+ plasmin (excess) ==> ( 2-antiplasmin-plasmin) + plasmin (residue). Substrate – RNA + plasmin (residue) ==> Peptide + RNA REACHROM- 2 ANTIPLASMIN The kit of reagents for the determination antiplasmin activity by photometric method. Cat. # FA-2 for 20 assays. Reagents: – Plasmin (4 mL) lyophilized \u2013 3 vials; – Chromogenic substrate (2 mL), lyophilized \u2013 1 vial; – Calibration plasma (1 mL), lyophilized \u2013 1 vial; – Concentrated buffer (10 mL) \u2013 2 vials. <\/p>\n","protected":false},"featured_media":4428,"template":"","meta":{"nf_dc_page":""},"product_brand":[],"product_cat":[206],"product_tag":[],"class_list":["post-4427","product","type-product","status-publish","has-post-thumbnail","product_cat-renam","cms-has-post-thumbnail","first","instock","shipping-taxable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product\/4427","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media\/4428"}],"wp:attachment":[{"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/media?parent=4427"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_brand?post=4427"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_cat?post=4427"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/mira-lab.com\/new\/wp-json\/wp\/v2\/product_tag?post=4427"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nThe kit of reagents for the determination antiplasmin activity<\/strong>
\nby photometric method.<\/strong>
\nCat. # FA-2 for 20 assays.
\nReagents:
\n– Plasmin (4 mL) lyophilized \u2013 3 vials;
\n– Chromogenic substrate (2 mL), lyophilized \u2013 1 vial;
\n– Calibration plasma (1 mL), lyophilized \u2013 1 vial;
\n– Concentrated buffer (10 mL) \u2013 2 vials.<\/p>\n