The HIB19 antibody reacts with CD19, an ~95 kDa type 1 transmembrane glycoprotein expressed on the surface of B cells throughout all stages of development, from early pre-B cells to plasma cells. Expression is down-regulated but persists in terminally differentiated plasma cells. CD19 is also found on follicular dendritic cells. By associating with CD21 and CD81, CD19 functions as a co-receptor for the B cell receptor and is involved in B cell activation and differentiation. Activation of CD19 is accompanied by phosphorylation of the cytoplasmic domain, which promotes binding to kinases and the induction of intracellular signaling cascades. Mutations in CD19 can result in severe immunodeficiency syndromes.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human CD19 Positive Selection Kit , EasySep™ Human Whole Blood CD19 Positive Selection Kit , EasySep™ HLA Whole Blood B Cell Positive Selection Kit ; partial blocking may be observed, as well as EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood and EasySep™ HLA Total Lymphocyte Enrichment: Complete Processing Kit for Whole Blood .