The TRA‐1‐60R antibody reacts with TRA-1-60 (Podocalyxin), a > 200 kDa pluripotent stem cell-specific protein expressed on the surface of undifferentiated human embryonic stem (ES) and induced pluripotent stem (iPS) cells, embryonal carcinoma (EC) cells and embryonic germ (EG) cells, as well as rhesus monkey ES cell lines. A soluble form of TRA-1-60 has been detected in serum of patients with embryonal carcinoma. The epitope, which is lost upon cell differentiation, contains sialic acid and is associated with a large-molecular-mass transmembrane protein named podocalyxin. Though sialylated, the epitope recognized by the TRA‐1‐60R antibody is resistant to treatment with neuraminidase.

This antibody clone has been verified for labeling human ES and iPS cells grown in TeSR™-E8™ , mTeSR™1 , and TeSR™2 , and for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human ES/iPS Cell TRA-1-60 Positive Selection Kit  and EasySep™ hESC/hiPSC SSEA-4 Positive Selection Kit.