The 2.4G2 antibody reacts with a shared epitope of Fc-gamma receptors CD16 (FcRIII) and CD32 (FcRII) in the mouse. CD16 and CD32 are low affinity receptors for the Fc portion of IgG, with CD32 having a higher affinity for IgG aggregates and immune complexes. CD16 and CD32 are expressed on B cells, monocytes, macrophages, NK cells, neutrophils, granulocytes, mast cells, and dendritic cells. The binding of immunoglobulins to Fc receptors is involved in multiple immunological functions, including phagocytosis, cytotoxocity, and the modulation of immune responses. The 2.4G2 antibody blocks the interaction between IgG and CD16 or CD32, and therefore is used as a pre-treatment to minimize non-specific binding of antibodies to FcR-bearing cells in applications such as flow cytometry, cell sorting, and immunofluorescence.