The 1A8 antibody reacts with Ly-6G, a 21 – 25 kDa GPI-anchored protein, which together with the structurally related Ly-6C protein comprises the granulocyte receptor-1 antigen (Gr-1). Gr-1 is expressed on monocytes, neutrophils and subsets of macrophages, plasmacytoid dendritic cells, and T cells. Monocytes in the bone marrow transiently express Gr-1 during development and the expression level is strongly correlated with granulocyte differentiation and maturation. In the periphery, Gr-1 is found predominantly on neutrophils and is a useful marker for these cells. The 1A8 antibody binds specifically to Ly-6G, whereas another commonly used antibody, clone RB6-8C5, binds to both Ly-6G and Ly-6C. It has been reported that the 1A8 antibody detects Ly-6G-expressing granulocytes in peripheral blood, whereas the RB6-8C5 antibody also binds to Ly-6C-expressing lymphocytes, monocytes, and dendritic cells.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Neutrophil Enrichment Kit