The MC-631 antibody reacts with a galactosylgloboside epitope on the stage-specific embryonic antigen-3 (SSEA-3), which is expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), undifferentiated embryonic stem (ES), and induced pluripotent stem (iPS) cells, as well as rhesus monkey ES cell lines. No immunoreactivity is evident with undifferentiated mouse EC, EG and ES cells. Expression of SSEA-3 is down regulated following differentiation of ES and EC cells. In contrast, the differentiation of mouse ES and EC cells may be accompanied by an increase in SSEA-3 expression.

This antibody clone has been verified for labeling human ES and iPS cells grown in TeSR™-E8™ , mTeSR™1 and TeSR™2 and has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human ES/iPS Cell TRA-1-60 Positive Selection Kit