The MC-813-70 antibody reacts with stage-specific embryonic antigen-4 (SSEA-4), a glycolipid carbohydrate antigen expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), undifferentiated embryonic stem (ES) and induced pluripotent stem (iPS) cells, a subset of mesenchymal stem cells, and rhesus monkey ES cell lines. No immunoreactivity is evident with undifferentiated mouse EC, EG, ES, and iPS cells. Expression of SSEA-4 is down-regulated following differentiation of human EC, ES, and iPS cells. In contrast, the differentiation of mouse EC, ES, or iPS cells may be accompanied by an increase in SSEA-4 expression.

This antibody clone has been verified for labeling human ES and iPS cells grown in mTeSR™1 , and TeSR™2, and has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human ES/iPS Cell TRA-1-60 Positive Selection Kit and EasySep™ hESC/hiPSC SSEA-4 Positive Selection Kit.