The MEL-14 antibody reacts with murine CD62L (L-Selectin or LECAM-1), an ~74 – 95 kDa type I glycoprotein expressed on the surface of a majority of leukocytes. CD62L mediates leukocyte-endothelial cell interactions through its association with ligands such as CD34, glyCAM-1, MAdCAM-1 and PSGL-1. It functions to facilitate lymphocyte rolling on the vascular endothelium, trafficking of lymphocytes to the lymph nodes, and homing of lymphocytes and neutrophils to sites of inflammation. Murine CD62L is expressed on most thymocytes, the highest expression levels being found on subsets of immunocompetent or dividing progenitor cells, as well as on neutrophils, eosinophils, monocytes, and subsets of B, T and NK cells. The MEL-14 antibody may be used together with antibodies to other cell surface markers to distinguish naïve, memory and effector T cells. The MEL-14 antibody has been shown to block migration of lymphocytes to lymph nodes and to inhibit leukocyte rolling.