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Krumdieck Tissue Slicer

The instrument provides samples of uniform and reproducible thickness, minimizes damage to surfaces of slices and thereby eliminates the main sources of error in tissue slice work. Slices from approximately 100 to 500 microns in thickness can be prepared at a maximum rate of one slice every 3 to 4 seconds. The slicer operates submerged in a buffer selected by the operator as most appropriate in terms of composition, tonicity, pH, temperature, oxygenation, and lubricating properties to maintain the viability of the tissues being sectioned. The microtome and the reservoir with the glass trap are sterilizable to allow the preparation of aseptic slices suitable for prolonged organ culture. The actual slicing is done by a rapidly reciprocating disposable blade oscillating at 1000 to 2500 rpm driven by a motor that also powers the impeller. The tissue slicer contains a cooling block which allows the temperature of the buffer solution in the slicer’s reservoir to be maintained.

Kim, K.H., Kim, J., Han, J.Y. et al. In vitro estimation of metal-induced disturbance in chicken gut-oviduct chemokine circuit. Mol. Cell. Toxicol. 15443–452 (2019).

Ramzan, A.A., Bitler, B.G., Hicks, D. et al. Adiponectin receptor agonist AdipoRon induces apoptotic cell death and suppresses proliferation in human ovarian cancer cells. Mol Cell Biochem 46137–46 (2019).

Fu, Y., Tong, J., Meng, F. et al. Ciliostasis of airway epithelial cells facilitates influenza A virus infection. Vet Res 4965 (2018).

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