Anti-Human CD45RO Antibody, Clone UCHL1 Mouse monoclonal IgG2a antibody against human, chimpanzee, common marmoset CD45RO

The UCHL1 antibody reacts with an extracellular epitope on CD45RO, which is the shortest isoform of CD45, a type I transmembrane glycoprotein and member of the protein tyrosine phosphatase family (receptor class 1/6 subfamily). Alternative splicing of exons 4, 5 and 6 that encode the extracellular RA, RB and RC polypeptides of CD45 gives rise to up to 8 isoforms with molecular masses of 180 – 240 kDa. Excision of all three exons generates the ~180 kDa CD45RO isoform, which is expressed on activated and memory (but not naïve) T cells, some B cell subsets, activated monocytes and macrophages, and granulocytes. CD45RO enhances both T cell receptor- and B cell receptor-mediated activation and is a known ligand for CD22 on B cells. The UCHL1 antibody has been used to identify T cell lymphomas and leukemia and is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. The proportion of CD45RO+ (memory) T cells typically increases with age. The UCHL1 epitope is destroyed by treatment with neuraminidase or O-glycosidase.