Overview
The 8e11 antibody reacts with stage-specific embryonic antigen-5 (SSEA-5), a terminal H type-1 glycan (Fuc1-2Galβ1-3GlcNAcβ1) expressed on the surface of cells in the inner cell mass of the human blastocyst during embryogenesis, and on undifferentiated human embryonic stem (ES) and induced pluripotent stem (iPS) cells. Expression of SSEA-5 is rapidly down-regulated upon cellular differentiation. The 8e11 antibody can be used in conjunction with other pluripotency surface markers such as CD9 and CD90, or CD50 and CD200, to sort and remove undifferentiated teratoma-forming cells from incompletely differentiated stem cell cultures.
Subtype: Primary Antibodies
Target Antigen: SSEA-5
Alternative Names: Stage-specific embryonic antigen-5
Reactive Species: Human
Conjugation: Alexa Fluor 488; APC; FITC; PE; Unconjugated
Host Species: Mouse
Cell Type: Pluripotent Stem Cells
Application: Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blotting
Area of Interest: Stem Cell Biology
Clone: 8e11
Gene ID: Not applicable
Isotype: IgG1, kappa
