Overview
The ALDHbr Assay Kit is optimized for the identification and quantitation of CD34+ cells that express high levels of the enzyme aldehyde dehydrogenase (ALDH) in human cord blood samples. This kit contains both the CD34+ Cell Detection Kit and the ALDEFLUOR™ Kit. The CD34+ Cell Detection Kit contains pre-selected fluorochrome-conjugated monoclonal antibodies that have been optimized for use with the ALDEFLUOR™ Kit to detect cells that express CD34 and have high ALDH activity by flow cytometry. The CD34+ Cell Detection Kit contains anti-human antibodies APC CD34, PE CD45, and PE-Cyanine5 Glycophorin A/B, and 7-AAD viability dye.
A flow cytometer equipped with a 488 nm laser and a 635 nm laser for excitation and appropriate filters for detecting APC, PE, PE-Cyanine5 and 7-AAD fluorescence is required for use.
Advantages:
• Quantifies primitive HSCs in < 1 day
• Optimized to detect ALDHbrCD34+ cells in CB
• Enumerates viable, metabolically active, primitive cells in the CD34+ cell population
• Optimized to detect ALDHbrCD34+ cells in CB
• Enumerates viable, metabolically active, primitive cells in the CD34+ cell population
Components:
ALDHbr Assay Kit (Catalog #01711)
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1 x ALDEFLUOR™ Kit (Catalog #01700)
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ALDEFLUOR™ Reagent
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ALDEFLUOR™ DEAB Reagent
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ALDEFLUOR™ Assay Buffer
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2 N HCl
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DMSO
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2 x CD34+ Cell Detection Kits
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7-AAD Viability Dye
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Anti-Human CD45 Antibody, Clone HI30, PE
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Anti-Human CD34 Antibody, Clone 581, APC
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Anti-Human CD235ab (Glycophorin AB) Antibody, Clone HIR2, PE-Cyanine5
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Application: Flow Cytometry
Area of Interest: Cord Blood Banking; Stem Cell Biology